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Focus: Understand spatiotemporal heterogeneity in semiconductor nanocrystals/heterostructures, soft-materials and biological systems using sub-ensemble and single-molecule fluorescence spectroscopy and diffusion dynamics
Systems of current interest to probe extent of heterogeneity:
- Carrier localization in (In,Ga)N quantum-wells (QWs) used for solid-state LEDs
- Luminescent quantum-dots (QDs), doped/alloyed nanocrystals and nanoaggregates
- Hybrid/doped perovskite micro-/nano-crystals for solar photovoltaics (PVs) and LEDs
- Soft matter hydrated networks/gels such as polymers/ionomers and polymersomes
- Spatial distribution of analytes (ions/molecules) in intracellular media (single-cells)
- Structural heterogeneity of amyloid fibrils relevant for neurodegenerative diseases
- Liquid liquid phase separation of proteins and peptides
- Biosensors
Techniques and methodologies used:
- Laser epifluorescence and total internal reflection fluorescence (TIRF) microscopy
- Single-emitter (molecule/nanocrystal/defect) spectroscopy and blinking (emission intermittency)
- Super-resolution single-molecule/particle tracking (translational and reorientational dynamics)
- Spectrally resolved fluorescence microscopy (spatially-resolved luminescence spectroscopy)
- Spectrally resolved sensitization imaging
- Polarization-resolved fluorescence anisotropy imaging
- Polarization-resolved single-molecule tracking (PR-SMT)
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence lifetime imaging microscopy (FLIM)
