C. P. Rao

    Professor

    Department of Chemistry

    Indian Institute of Technology Bombay

    Powai, Mumbai 400076

    Ph:   022-2576 7162

    Fax:  022-2576 7152

    Email: cprao[at]chem.iitb.ac.in

    Group Webpage

Academic Background

B.Sc. Andhra Univ. Chemistry as main, and physics and Walter mathematics as ancillaries

M.Sc. IIT, Madras Inorganic, Organic, Physical and Theoretical

Ph.D. IISc., Bangalore Inorganic, Bioinorganic & Biophysical

 

Professional Experience

Assistant Professor ,Indian Institute of Technology Bombay,1988-1994

Associate Professor ,Indian Institute of Technology Bombay, 1994-1998

Professor, Indian Institute of Technology Bombay, 1998-

Research Interests

 

Small molecular systems proteins enzymes and nucleic acid Molecular Dynamics Simulations
Conjugates of calixarenes & carbohydrates possessing variety of binding cores: 

Organic Synthesis; Coordination & Structural aspects; Structural & Functional Biomimetics for metalloproteins & metalloenzymes; As Metal Ion & Molecular SENSORS

(Absorption, emission and mass spectrometric studies; Steady state and time resolved fluorescence spectroscopy)

Computational Evaluation of the structure and energetics of the species of recognition.

Nano-structural features of the simple receptors and their species of recognition by AFM, TEM  & SEM

Molecular aggregation by Dynamic Light Scattering (DLS)

Lectin & Glycosidases:   Isolation and purification

Binding, property modification & inhibition of LECTINS  & GLYCOSIDASES by glyco-conjugates:   Assay, Fluorescence, CD & ITC & Microscopy (AFM, SEM & TEM) 

Metal ion complexes as Artificial Chemical Nucleases (DNA Cleavage studies)

Computational docking studies of the conjugates of carbohydrates & calixarenes with lectins and glycosidases

Metal ion up-take by apo-proteins

Lectin glycobiology:     Biological aspects of lectins and their conjugates

 

 Building artificial metalloenzymes  ---  with specific reference to Zinc Enzymes

 

 

Representative Publications

  • R. K. Pathak, V. K. Hinge, M. Mondal and C. P. Rao, "Triazole-linked-thiophene conjugate of calix[4]arene: Its selective recognition of Zn2+ and as biomimetic model in supporting the events of the metal detoxification and oxidative stress involving metallothionein" J. Org. Chem., 2011, (Accepted), DOI:10.1021/jo201865x

  • . K. Pathak, K. Tabbasum, V. K. Hinge, and C. P. Rao, "Selective recognition of cysteine in its free and protein bound states by the Zn2+ complex of triazole based calix[4]arene conjugate" Chem. Eur. J., 2011, (Accepted), DOI: 10.1002/chem.201102791

  • A. Bandela, J. P. Chinta and C. P. Rao, "Role of the conformational changes brought in the arms of 1,3-Di-capped conjugate of calix[4]arene (L) in turning on the fluorescence of L by Hg2+" Dalton Trans. 40(2011), 11367-11370. DOI:10.1039/C1DT11208B

  • A. Kumar, J. P. Chinta, A. K. Ajay, M. K. Bhat and C. P. Rao, "Synthesis, characterization, plasmid cleavage and cytotoxicity of cancer cells by a copper(II) complex of anthracenyl-terpyridine" Dalton Trans., 40(2011) 10865-10872. DOI:10.1039/C1DT10201J (Hot article)

  • R. Joseph, J. P. Chinta and C. P. Rao, "Calix[4]arene-Based 1,3-Diconjugate of Salicylyl Imine Having Dibenzyl Amine Moiety (L): Synthesis, Characterization, Receptor Properties toward Fe2+, Cu2+, and Zn2+, Crystal Structures of Its Zn2+ and Cu2+ Complexes, and Selective Phosphate Sensing by the [ZnL]" Inorg. Chem., 50(2011) 7050-7058. DOI:10.1021/ic200544a

  • R. Joseph and C. P. Rao, "Ion and Molecular Recognition by Lower Rim 1,3-Di-conjugates of Calix[4]arene as Receptors", Chem. Rev., 111(2011) 4658-4702, DOI:10.1021/cr1004524

  • A. Mitra, V. K. Hinge, A. K. Mittal, S. Bhakta, P. Guionneau and C. P. Rao, "1-(D-Glucopyranosyl-2'-deoxy-2'-iminomethyl)-2-hydroxy-naphthalene conjugate (L): Sensing Zn2+ followed by phosphate including DNA". Chem. Eur. J., 11(2011) 8044-8047 DOI: 10.1002/chem.201100734.

  • A. Bandela, J. P. Chinta, V. K. Hinge, A. Dikundwar, T. N. Guru Row and C. P. Rao, "Recognition of polycyclic aromatic hydrocarbons and their derivatives by 1,3-di-naphthalimide conjugate of calix[4]arene: Emission, absorption, crystal structures and computational studies", J. Org. Chem., 76(2011) 1742-1750. DOI: 10.1021/jo1023409 .

  • A. Mitra, A. K. Mittal and C. P. Rao, "Carbohydrate assisted fluorescence turn-on gluco-imino-anthracenyl conjugate as Hg(II) sensor in milk and blood serum milieu", Chem. Comm , 47(2011) 2565-2567 DOI: 10.1039/C0CC04967K)

  • A. Acharya, B. Ramanujam, J. P. Chinta, and C. P. Rao, "1,3-Diamido-calix[4]arene Conjugates of Amino Acids: Recognition of ?COOH Side Chain Present in Amino Acids, Peptides, and Proteins by Experimental and Computational Studies", J. Org. Chem., 76 (2010) 127-137. DOI: 10.1021/jo101759f.

  • A. Kumar, B. Ramanujam, N. K. Singhal, A. Mitra and C. P. Rao, "Interaction of aromatic-imino-glycoconjugates with jacalin: Experimental and computational docking studies", Carbohydr. Res., 345 (2010) 2491-2498. DOI: 10.1016/j.carres.2010.09.014

  • A. Acharya, B. Ramanujam, A. Mitra and C. P. Rao, "Nano-Fibers Formed Through Stacking of the Complexes of Glucosyl-C2-Salicyl-Imine and Phenylalanine: Characterization by Microscopy, Modeling by Molecular Mechanics and Interaction by -Helical & -Sheet Proteins", ACS Nano, 4 (2010) 4061-4073. DOI: 10.1021/nn1006286.

  • R. K. Pathak, A. G. Dikundwar, T. N. Guru Row and C. P. Rao, "A lower rim triazole linked calix[4]arene conjugate as a fluorescence switch on a sensor for Zn2+ in blood serum milieu", Chem. Comm., 46 (2010) 4345-47. DOI: 10.1039/c0cc00219d.

  • R. Joseph, J. P. Chinta and C. P. Rao, "Benzothiazole appended lower rim 1,3-di-amido-derivative of calix[4]arene: Synthesis, structure, receptor properties towards Cu2+, iodide recognition and computational modelling", Inorg. Chimica acta (2010) 2833-2839. DOI: 10.1016/j.ica.2010.04.005.

  • R. Joseph, J. P. Chinta and C. P. Rao, "Lower rim 1,3-di-derivative of calix[4]arene appended salicylidene imine (H2L): Experimental and computational studies of the selective recognition of H2L towards Zn2+, and sensing phosphate and amino acid by [ZnL]", J. Org. Chem., 75 (2010) 3387-3395. DOI: 10.1021/jo1004247

  • M. Dey, A. Ali, A. Acharya & C. P. Rao, "1,3-Di-peptido-conjugates of calix[4]arene and its di-OCH3 derivatives: Synthesis, characterization and phosphate recognition", Indian J. Chem. 49B (2010) 1098-1108.

  • A. Ali, R. Joseph, B. Mahieu and C. P. Rao, "Synthesis and characterization of a (1+1) cyclic Schiff base of lower rim 1,3-diderivative of p-tert-butylcalix[4]arene and its complexes of VO2+, UO22+, Fe3+, Ni2+, Cu2+ and Zn2+", Polyhedron 29 (2010) 1035-1040. DOI: 10.1016/j.poly.2009.12.020.

  • A. Mitra, J.P. Chinta and C.P. Rao, "1-(D-Glucopyranosyl-2´-deoxy-2´-iminomethyl)-2-hydroxybenzene as chemosensor for aromatic amino acids by switch-on fluorescence", Tetrahedron Lett. 51 (2010) DOI:10.1016/j.tetlet.2009.10.105

  • R. Joseph, B. Ramanujam, A. Acharya and C.P. Rao, "Lower rim 1,3-di-{bis-(2-picolyl)}amide derivative of calix[4]arene (L) as ratiometric primary sensor towards Ag+ and the complex of Ag+ as secondary sensor towards Cys: Experimental, Computational and Microscopy studies, and INHIBIT Logic Gate Properties of L", J. Org. Chem.74 (2010) DOI: 10.1021/jo901676s

  • M. Dey, J.P. Chinta, G.J. Long and C.P. Rao, "Synthesis and characterization of the complexes of Fe(III), Co(III), Ni(II), Cu(II), Zn(II) and UO22+ with p-tert-butylcalix[4]arene bearing two imine pendants linked through salicylyl moiety at the lower rim", Indian J. Chem. 48A (2009) 1484-1491

  • G.S. Baghel and C.P. Rao, "Pamoic acid in forming metallo-organic framework: Synthesis, characterization and first crystal structure of a dimeric Ti(IV) complex", Polyhedron 28 (2009) 3507-14. DOI: 10.1016/j.poly.2009.07.050

  • R. Joseph, Balaji R., A. Acharya and C.P. Rao, "Fluorescence Switch-on Sensor for Cu2+ by an Amide Linked Lower Rim 1, 3 -bis(2-picolyl)amine Derivative of Calix[4]arene in Aqueous Methanol", Tetrahedron Letts., 50 (2009) 2735-2739.DOI: 10.1016/j.tetlet.2009.03.109

  • Jugun, P. C., A. Acharya, A. Kumar and C.P. Rao, Spectroscopy and Microscopy Studies of the Recognition of Amino Acids and Aggregation of Proteins by Zn(II)-Complex of Lower Rim Naphthylidene Conjugate of Calix[4]arene, J. Phys. Chem B 113, (Revision 2009) 12075-83. DOI: 10.1021/jp903099b

  • N.K. Singhal, A. Mitra, G. Rajsekhar, M.M. Shaikh, Subodh Kumar, P. Guionneau and C.P. Rao, Role of the orientation of OH groups on sensitivity and selectivity of the interaction of M2+ with ribosyl- and galctosyl-imino-conjugates: Solution recognition studies of M2+ in MeOH and selective recognition of Cu2+ in HEPES buffer, and first crystal structure determination of dinuclear-Cu(II) complexes based on both the glyco-imino-conjugates, Dalton Transactions (Revision, 2009).8432-42. DOI: 10.1039/b903668g

  • R. Joseph, B. Ramanujam, A. Acharya and C.P. Rao, Fluorescence Switch-on Sensor for Cu2+ by an Amide Linked Lower Rim 1, 3 -bis(2-picolyl)amine Derivative of Calix[4]arene in Aqueous Methanol, Tetrahedron Letts., 50 (2009) 2735-2739. DOI: 10.1016/j.tetlet.2009.03.109

  • R.K. Pathak, Sk.Md. Ibrahim and C.P. Rao, Selective recognition of Zn2+ in aqueous acetonitrile by salicylaldimine appended triazole linked di-derivatives of calix[4]arene by enhanced fluorescence emission: Role of terminal CH2OH moieties in conjunction with the imine in recognition, Tetrahedron Letts., 50 (2009) 2730-2734. DOI: 10.1016/j.tetlet.2009.03.126

  • A. Kumar, N.K. Singhal, B. Ramanujam, A. Mitra, N.R. Rameshwaram, S.K. Nadimpalli and C.P. Rao, C1-/C2-aromatic-imino-glyco-conjugates: Experimental and computational studies of binding, inhibition and docking aspects towards glycosidases isolated from soybean and jack bean, Glycoconjugates J. 26(4) (2009) 495-510. DOI: 10.1007/s10719-008-9199-4

  • G.S. Baghel, S.M. Shaikh and C.P. Rao, Metal ion complex of di-O-picolyl derivative of 1,1'methylene-bis(2-naphthol): First crystal structure of a monomeric Cu(II) complex of bis(2-((pyridin-2-yl)methoxy)naphthalen-1-yl)methane, Inorg. Chim. Acta. 362 (2009) 2770-2775. DOI: 10.1016/j.ica.2008.12.019

  • A. Mitra, B. Ramanujam, and C.P. Rao, 1-(D-glucopyranosyl-2'-deoxy-2'-iminomethyl)-2-hydroxynaphthalene as chemo-sensor for Fe3+ in aqueous HEPES buffer based on colour changes observable by naked eye, Tetrahedron. Letts., 50 (2009) 776-780. DOI: 10.1016/j.tetlet.2008.11.116

  • G.S. Baghel, B. Ramanujam and C.P. Rao, Selective Recognition of Cu2+ by Di-O-picolyl Derivative of 1,1'Methylene-Bis(2-Naphthol) J. Photochem. Photobiol. A, 202 (2009) 172-177. DOI: 10.1016/j.jphotochem.2008.11.021

  • R. Joseph, B. Ramanujam, H. Pal and C.P. Rao, Lower rim 1,3-di-derivative of calix[4]arene possessing bis-{N-(2-2'-dipyridylamide)} pendants: An unusual dual fluorescence sensor for Zn2+ and Ni2+, Tetrahedron. Letts., 49 (2008) 6257-61. DOI:10.1016/j.tetlet.2008.08.049

  • R. Joseph, B. Ramanujam, A. Acharya, A. Khutia and C.P. Rao, Experimental and Computational Studies of Selective Recognition of Hg2+ by Amide Linked Lower Rim 1, 3-di-benzimidazole Derivative of Calix[4]arene: Species Characterization in Solution and that in the Isolated Complex, Including the Delineation of the Nano-Structures, J. Org. Chem. 73 (2008) 5745-58. DOI:10.1021/jo800073g

  • R. Ahuja, N.K. Singhal, B. Ramanujam, M. Ravikumar, and C.P. Rao, Experimental and Computational Studies of the Recognition of Amino Acids by Galactosyl-imine and -amine Derivatives: An Attempt to Understand the Lectin-Carbohydrate Interactions, J. Org. Chem., 72 (2007) 3430-3442. DOI: 10.1021/jo0700979

  • N.K. Singhal, B. Ramanujam, V. Mariappandar and C.P. Rao, Carbohydrate-Based Switch-On Molecular Sensor for Cu(II) in Buffer: Absorption and Fluorescence Study of the Selective Recognition of Cu(II) Ions by Galactosyl Derivatives in HEPES Buffer, Org. Lett., 8 (2006) 3525-28. DOI: 10.1021/ol061274f.