CH 518 : Macromolecular Crystallography


Basic Diffraction Theory, Bragg’s law, Miller Indices, Laue Equations, Protein and Nucleic acid Structure, X-ray major sources and production, Xray detectors, Crystallization techniques and principles, symmetry and space groups, reciprocal space, Fourier transform, structure factor equation, phase problem, data collection and processing, methods of structure determination, heavy atom solutions like direct methods, patterson methods, Multiple Anomalous diffraction, Single Anomalous diffraction, sulpur phasing, Isomorphous replacement, Molecular replacement, structure refinement and validation, structure deposition, elucidation of mechanism from structure, biological crystallography examples of virus, ribosomes, membrane proteins, macromolecular assemblies

Text References

  1. Principles of protein x-ray crystallography Jan Drenth,
  2. Crystallography Made Crystal Clear , Gale Rodes
  3. Structure determination by X-ray crystallography M. Ladd and  R Palmer