Make and Model: 
Bruker , Autoflex Max
Description: 
MALDI-Time-Of-Flight Mass Spectrometer for accurate mass determination and structure identification of bio-molecules. Includes MALDI Perpetual™ ion source with self-cleaning device, extended pulsed ion extraction PAN™ mass range focusing; Bruker smart beam™-II laser technology for versatile applications with different sample preparation techniques; TOF analyser for linear and reflection measurements in positive/negative ion mode with integrated FlashDetector™ systems; MS/MS capability based on LIFT™ TOF/TOF technology; high-energy CID (Collision-Induced Dissociation) accessory Data System with 10 bit 5 Gs/s digitizer, WIN10 Operating-System, >=24| Widescreen-LCD-Display and Compass™ for Flex software package for MS control, data acquisition and processing
Features: 
BioTools software allows the identification and detailed characterization of proteins and peptides using various types of mass spectra. This includes database search of single spectra and combined MS and MS/MS datasets, the import of search results and further detection of modifications and mutations. The RapiDeNovo module provides extensive de-novo sequencing capabilities. BioTools also supports various top-down sequencing spectra types for intact protein characterization and enables the annotation and database search of these data. PolyTools™ 2.0 is a program for the interpretation of MALDI mass spectra of polymers. It accepts peak lists and automatically detect the repeat unit, cation and end groups upon loading of the lists. It also calculates characteristic parameters of the chain length distribution like |molecular weight average|, |polydispersity| or |degree of polymerization|. It displays the results in tabular and graphical representation and can export both for further use in other programs. Kendrick Mass Defect (KMD) analysis of the data is possible as well and a variety of KMD analysis types/plots is available (KMD, KMD-RNKM, RKM and RKM-RNKM). smartbeam II (DPSS) Used in autoflex max MALDI TOF TOF. The smartbeam I components (modulator, focus lens, pin hole) are inside the laser housing. Wave length : 355 nm (diode pumped solid state laser) Pulse duration : > 3ns Pulse energy : < 100µJ Repetition rate : 2000 Hz Beam diameter: smartbeam pattern Divergence : smartbeam pattern Lifetime : 3.5 x109 shoots Power supply : 100 - 240 V
Location: 

Room No 370, Second floor, Central Facility Room, Chemistry Building

Contact details: 
Email: rajeshg@chem.iitb.ac.in , Contact Phone # – 022 2576 4159
Faculty Incharge: 

Prof. Chidambar Kulkarni (chidambark@chem.iitb.ac.in)