CH 818

Single Molecule Fluorescence Spectroscopy
Syllabus: 

Methods: Fundamentals of fluorescence microscopy and benefits of probing the sub-ensemble; Requirements and evidences for single-molecule/nanocrystal fluorescence detection; Advantages/drawbacks of various single-molecule (SM) imaging techniques; SM image processing, data analyses procedures and interpretation. Dynamics and Spectroscopy: Single-emitter fluorescence intermittency (blinking); SM diffusion (translational and rotational) dynamics; Spectroscopy of single emitters; SM fluorescence lifetime; single-emitter energy and electron transfer. Applications: Select applications of in chemistry, soft matter systems, biology/biophysics and nanomaterials science through discussion of seminal publications.

Text References: 

1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd Ed, Springer, 2007.

2. D. B. Murphy, Fundamental of Light Microscopy and Electronic Imaging, Wiley-LISS, 2001.

3. C. Gell, D. Brockwell and A. Smith, Eds. Handbook of Single Molecule Fluorescence Spectroscopy, Oxford, 2013.

4. M. Sauer, J. Hofkens and J. Enderlein, Eds. Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules, Wiley-VCH, 2010.

5. C. Brauchle, D.C. Lamb and J. Michaelis Eds. Single Particle Tracking and Single Molecule Energy Transfer, Wiley-VCH, 2010.