Single-Molecule Spectroscopy (SMS) is a technique where the fluorescence emission from spatially isolated molecules are collected and analyzed over a course of time in order to retrieve new information about individual molecules in a condensed media. Using SMS, rare events that are normally obscured by ensemble measurements can be observed and the inhomogeneity in specific molecular properties can be evaluated. In the past decade, SMS has been shown to be a very powerful tool to elucidate complex mechanistic pathways and a unique probe for the local nano-environment around isolated molecule. Our plan is to take a multidisciplinary approach towards the detection and imaging of Single-Molecules (SM) that would incorporate physical chemistry with nano-materials and biomolecular sciences. We plan to focus on research fields related to fluorescent behaviors of Single-Molecules or their Aggregates in unique local environments in contrast to that of bulk. Using a specialized technique called Total Internal Reflection Fluorescence Microscopy (TIRFM) as an experimental tool, we want to visualize and study real-time dynamic processes occurring near the interface to answer fundamental questions pertinent to chemistry, biology, polymer science and nano-materials.